P-366 Unraveling the whole transcriptome profiles of receptive phase endometrium in infertile women

Abstract Study question Are there differential transcriptomic profiles of the receptive phase endometria in infertile women with different infertility diagnoses? Summary answer Endometrium-related pathologies such as endometriosis and RIF seemed to share similar endometrial molecular mid-secretory phase,while samples from unexplained were controls. What is known already Successful embryo implantation orchestrated by combination a viable blastocyst, endometrium perfectly synchronized dialogue between them. Endometrium-associated recurrent failure (RIF) are suggested hamper receptivity, but mechanisms affected these other diagnoses need further investigation. Previous transcriptome studies human have analysed separately, whether what extent receptive-phase vary distinct underexplored. design, size, duration A prospective, cross-sectional study was performed at Reproductive Unit University Hospital March 2019 April 2021. Forty-five well-characterized (age 34.7±3.8 years, BMI 24.5±kg/m2) recruited before starting any treatment. Four groups according established: (n = 12), 14), 10), male factor control group 9). Participants/materials, setting, methods Endometrial biopsies collected using Pipelle curette during (LH + 7) natural cycle. Total RNA extracted sequenced Illumina NovaSeq 6000 technology. Raw sequencing data quality assessed, pre-processed quantified, expression enrichment analyses R software. Main results role chance Differential analysis revealed 743, 587 140 differentially expressed genes (DEGs) endometriosis, infertility, respectively, when compared group. Among these, 31 exhibited consistent all groups. Regarding functional analyses, detected DEGs mainly related immune response inflammatory processes, supporting involvement pathways impairment receptivity. Interestingly, comparison showed endometrium-associated RIF, while had infertility. These indicate common receptivity patients. Limitations, reasons for caution Further research bigger sample size required confirm findings. Wider implications findings We identified associated diagnoses. Further, tended women, meaning that factors than issues could lead their Our helps understand underlying female Trial registration number not applicable